Pcyt1b Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:84309 Karim M, et al., Gene structure, expression and identification of a new CTP:phosphocholine cytidylyltransferase beta isoform. Biochim Biophys Acta. 2003 Jul 4;1633(1):1-12
Assay type:	Western blot
MGI Accession ID:	MGI:2671330
Gene symbol:	Pcyt1b
Gene name:	phosphate cytidylyltransferase 1, choline, beta isoform

Antibody:	anti-CCT beta
Detection system:	Biotinylated secondary antibody/[Strept]avidin
Assay notes:	This experiment was repeated three times.

Results

Image: 7_right

	Sample Information		Bands			Other Sample Information
Lane	Age	Structure	47 kDa (a)	43 kDa (b)	39 kDa (c)	Genetic Background	Mutant Allele(s)	Sex	Note
Brain	P	TS28: brain	Present	Present	Present	Not Specified		Not Specified
Liver	P	TS28: liver	Absent (d)	Ambiguous (e)	Absent (d)	Not Specified		Not Specified	The authors stated that the liver lysate did not display a strong signal for either isoform.

Notes:
(a) This is band is probably comprised of the phosphorylated forms of the proteins.
(b) This is the beta two isoform.
(c) This is the beta three isoform.
(d) Absent assigned by curator.
(e) It was unclear whether this band represents low level expression of the beta two isoform or whether it was due to nonspecific antibody binding.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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