Gene Expression Data
Assay Details
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Assay
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Reference:
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J:55762
Montgomery RA, et al., Analysis of cytokine gene expression during fetal thymic ontogeny using the polymerase chain reaction. J Immunol. 1991 Jul 15;147(2):554-60
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:2670108
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Gene symbol:
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Il1a
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Gene name:
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interleukin 1 alpha
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Probe:
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IL-1a-pA, IL-1a-pB
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Probe preparation:
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labelled with P32 DNA
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Assay notes:
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The results of this assay were presented in Table I. The RT-PCR reaction was electrophoresed, Southern blotted and probed with an end-labeled oligonucleotide probe (MGI:2670084) internal to the amplified fragment. The probe used in this assay was5' labelled.
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Results
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| E14.5
(a)
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TS22: thymus primordium
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Absent
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1 µg; total RNA |
either: BALB/c or C57BR |
| Pooled |
| E14.5
(a)
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TS22: heart
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Absent
|
1 µg; total RNA |
either: BALB/c or C57BR |
| Pooled |
| E14.5
(a)
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TS22: liver
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Present
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1 µg; total RNA |
either: BALB/c or C57BR |
| Pooled |
| E15.5
(a)
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TS23: thymus primordium
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Absent
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1 µg; total RNA |
either: BALB/c or C57BR |
| Pooled |
| E15.5
(a)
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TS23: heart
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Absent
|
1 µg; total RNA |
either: BALB/c or C57BR |
| Pooled |
| E15.5
(a)
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TS23: liver
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Present
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1 µg; total RNA |
either: BALB/c or C57BR |
| Pooled |
| E16.5
(a)
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TS24: thymus
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Present
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1 µg; total RNA |
either: BALB/c or C57BR |
| Pooled |
| E16.5
(a)
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TS24: heart
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Absent
|
1 µg; total RNA |
either: BALB/c or C57BR |
| Pooled |
| E16.5
(a)
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TS24: liver
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Present
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1 µg; total RNA |
either: BALB/c or C57BR |
| Pooled |
| E17.5
(a)
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TS25: thymus
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Present
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1 µg; total RNA |
either: BALB/c or C57BR |
| Pooled |
| E17.5
(a)
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TS25: heart
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Absent
|
1 µg; total RNA |
either: BALB/c or C57BR |
| Pooled |
| E17.5
(a)
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TS25: liver
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Present
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1 µg; total RNA |
either: BALB/c or C57BR |
| Pooled |
| E18.5
(a)
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TS26: thymus
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Present
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1 µg; total RNA |
either: BALB/c or C57BR |
| Pooled |
| E18.5
(a)
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TS26: heart
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Absent
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1 µg; total RNA |
either: BALB/c or C57BR |
| Pooled |
| E18.5
(a)
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TS26: liver
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Present
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1 µg; total RNA |
either: BALB/c or C57BR |
| Pooled |
| E19.5
(a)
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TS26: thymus
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Present
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1 µg; total RNA |
either: BALB/c or C57BR |
| Pooled |
| E19.5
(a)
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TS26: heart
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Absent
|
1 µg; total RNA |
either: BALB/c or C57BR |
| Pooled |
| E19.5
(a)
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TS26: liver
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Present
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1 µg; total RNA |
either: BALB/c or C57BR |
| Pooled |
| E20.0
(a)
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TS26: thymus
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Present
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1 µg; total RNA |
either: BALB/c or C57BR |
| Pooled |
| E20.0
(a)
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TS26: heart
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Absent
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1 µg; total RNA |
either: BALB/c or C57BR |
| Pooled |
| E20.0
(a)
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TS26: liver
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Present
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1 µg; total RNA |
either: BALB/c or C57BR |
| Pooled |
Notes:
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