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Gene Expression Data
Assay Details
Assay
Reference: J:63767 Fraboulet S, et al., Expression of DM-GRASP/BEN in the developing mouse spinal cord and various epithelia. Mech Dev. 2000 Jul;95(1-2):221-4
Assay type: RNA in situ
MGI Accession ID: MGI:2669741
Gene symbol: Alcam
Gene name: activated leukocyte cell adhesion molecule
Probe: BEN cDNA
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 1A: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: neural tube floor plate Present 1A
TS15: dorsal root ganglion Present Regionally restricted 1A Expression is in neural crest cells located on either side of the neural tube that condense to form the dorsal root ganglion.

Specimen 1B: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: neural tube floor plate Strong 1B
TS17: future spinal cord Present Regionally restricted 1B Expression is in motor neurons.

Specimen 1C: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: neural tube floor plate Strong 1C
TS19: future spinal cord Strong Regionally restricted 1C Expression is in motor neurons.

Specimen 1D: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: spinal cord floor plate Strong 1D
TS20: spinal cord Strong Regionally restricted 1D Expression is in motor neurons.

Specimen 1E, Br: embryonic day 12.5 (more )
Note: Section is at the brachial level. Double labeled: blue - Alcam (BEN); red - Isl1 (assay MGI:2669778).
Structure Level Pattern Image Note
TS20: spinal cord floor plate Present 1E
TS20: spinal cord Present Spotted 1E Expression is present in a subset of motor neurons at the brachial level of the spinal cord.

Specimen 1F, Lb: embryonic day 12.5 (more )
Note: Section is at the lumbar level. Double labeled: blue - Alcam (BEN); red - Isl1 (assay MGI:2669778).
Structure Level Pattern Image Note
TS20: spinal cord floor plate Present 1F
TS20: spinal cord Present Spotted 1F Expression is present in a subset of motor neurons at the lumbar level of the spinal cord.

Specimen 1G, Cerv: embryonic day 12.5 (more )
Note: Section is at the cervical level. Double labeled: blue - Alcam (BEN); red - Isl1 (assay MGI:2669778).
Structure Level Pattern Image Note
TS20: spinal cord floor plate Present 1G
TS20: spinal cord Present Regionally restricted 1G Expression is in all motor neurons at the cervical level of the spinal cord.

Specimen 2A: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: spinal cord floor plate Present 2A
TS21: dorsal root ganglion Present 2A
TS21: axial skeleton Present Regionally restricted 2A Expression is in the periosteum.

Specimen 2B: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: dorsal root ganglion Present Spotted 2B

Specimen 2C: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: trigeminal ganglion Present Uniform 2C

Specimen 2D: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: vestibulo-cochlear ganglion Present Uniform 2D
TS21: cochlear duct epithelium Present 2D

Specimen 3A: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: nasal cavity epithelium Present 3A

Specimen 3B: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: pharynx epithelium Present 3B

Specimen 3C: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: main bronchus epithelium Present 3C

Specimen 3D: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: vibrissa Present Regionally restricted 3D Expression is in the hair placode.

Specimen 3E: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: vibrissa epidermal component Present Regionally restricted 3E Expression is in the central cells of the hair peg.

Specimen 3F: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: vibrissa epidermal component Present Regionally restricted 3F Expression is in the central cells of the hair peg.

Specimen 3G: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: vibrissa epidermal component Present Regionally restricted 3G Expressing cells form a plug at the skin surface and are pushed against the outer root sheath.

Specimen 3H: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: vibrissa epidermal component Present Regionally restricted 3H Expressing cells form a plug at the skin surface and are pushed against the outer root sheath.
TS24: vibrissa dermal component Present Single cells 3H Expression in some dermal cells and hair matrix.

Specimen Not Shown: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: spinal cord floor plate Present
TS23: dorsal root ganglion Present


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory