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Gene Expression Data
Assay Details
Assay
Reference: J:47884 Janne PA, et al., Functional overlap between murine Inpp5b and Ocrl1 may explain why deficiency of the murine ortholog for OCRL1 does not cause Lowe syndrome in mice. J Clin Invest. 1998 May 15;101(10):2042-53
Assay type: RT-PCR
MGI Accession ID: MGI:2668831
Gene symbol: Inpp5b
Gene name: inositol polyphosphate-5-phosphatase B
Probe: Inpp5b-pC, Inpp5b-pD
Probe preparation: labelled with P32
Visualized with: Autoradiography
Assay notes: The RT reaction was performed with samples containing a 1:1 mixture of wild type and mutant RNA.
Results
Image: 10 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex
Brain -RT Control
Brain 1 P adult TS28: brain Present Present (c) 2 µg; total RNA either: (involves: 129S6/SvEvTac * C57BL/6) or (involves: 129S6/SvEvTac * NIH Black Swiss) Inpp5btm1Nbm/Inpp5btm1NbmNot Specified
Brain 2 P adult TS28: brain Present Present (c) 2 µg; total RNA either: (involves: 129S6/SvEvTac * C57BL/6) or (involves: 129S6/SvEvTac * NIH Black Swiss) Inpp5btm1Nbm/Inpp5btm1NbmNot Specified
Kidney -RT Control
Kidney 1 P adult TS28: metanephros Present Weak (d) 2 µg; total RNA either: (involves: 129S6/SvEvTac * C57BL/6) or (involves: 129S6/SvEvTac * NIH Black Swiss) Inpp5btm1Nbm/Inpp5btm1NbmNot Specified
Kidney 2 P adult TS28: metanephros Present Weak (d) 2 µg; total RNA either: (involves: 129S6/SvEvTac * C57BL/6) or (involves: 129S6/SvEvTac * NIH Black Swiss) Inpp5btm1Nbm/Inpp5btm1NbmNot Specified
Spleen -RT Control
Spleen 1 P adult TS28: spleen Present Weak (d) 2 µg; total RNA either: (involves: 129S6/SvEvTac * C57BL/6) or (involves: 129S6/SvEvTac * NIH Black Swiss) Inpp5btm1Nbm/Inpp5btm1NbmNot Specified
Spleen 2 P adult TS28: spleen Present Weak (d) 2 µg; total RNA either: (involves: 129S6/SvEvTac * C57BL/6) or (involves: 129S6/SvEvTac * NIH Black Swiss) Inpp5btm1Nbm/Inpp5btm1NbmNot Specified
Liver -RT Control
Liver 1 P adult TS28: liver Present Weak (d) 2 µg; total RNA either: (involves: 129S6/SvEvTac * C57BL/6) or (involves: 129S6/SvEvTac * NIH Black Swiss) Inpp5btm1Nbm/Inpp5btm1NbmNot Specified
Liver 2 P adult TS28: liver Present Weak (d) 2 µg; total RNA either: (involves: 129S6/SvEvTac * C57BL/6) or (involves: 129S6/SvEvTac * NIH Black Swiss) Inpp5btm1Nbm/Inpp5btm1NbmNot Specified
Notes:
(a) This band represents the wild type band.
(b) This band represents the mutant band (30bp smaller than wild type).
(c) There appear to be equal amounts of the normal and mutant products.
(d) There is a markedly reduced amount of mutant product compared to wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
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