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Gene Expression Data
Assay Details
Assay
Reference: J:84601 Murer-Orlando M, et al., Differential regulation of gamma-crystallin genes during mouse lens development. Dev Biol. 1987 Jan;119(1):260-7
Assay type: Nuclease S1
MGI Accession ID: MGI:2668485
Gene symbol: Crygf
Gene name: crystallin, gamma F
Probe: gamma-2 probe2
Probe preparation: DNA
Visualized with: Autoradiography
Assay notes: The probe used in this assay was 5' labelled.
Results
Image: 4 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (b) Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex Note
Size markers Mol. Wt. Marker Lane
Size markers Mol. Wt. Marker Lane
No RNA Control
15d Embryo E15.0 (c) TS23: head Present Not Specified Not Specified 25 µg; total RNA Swiss Webster Not Specified For 15-day-old embryos, heads were decapitated and the bodies discarded.
1-day P1 TS27: eye Present Not Specified Not Specified 25 µg; total RNA Swiss Webster Not Specified
10-days P10 TS28: eye Present Not Specified Not Specified 25 µg; total RNA Swiss Webster Not Specified
40-days P40 TS28: eye Present Weak Weak 25 µg; total RNA Swiss Webster Not Specified
Notes:
(a) Predominant S1-resistant species at +1 to +10 position.
(b) Additional species at approximately -90 and -110.
(c) Age of embryo at noon of plug day not specified in reference.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory