Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	H1f3tm2Ais
Name:	H1.3 linker histone, cluster member; targeted mutation 2, Arthur I Skoultchi
MGI ID:	MGI:2666743
Synonyms:	H1d-
Gene:	H1f3  Location: Chr13:23739206-23739981 bp, + strand  Genetic Position: Chr13, 9.79 cM, cytoband A2-A3
Alliance:	H1f3tm2Ais page

Germline Transmission:	Earliest citation of germline transmission: J:83954
Parent Cell Line:	WW6 (ES Cell)
Strain of Origin:	STOCK 129/Sv and C57BL/6J and SJL

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: The coding region of the gene was replaced with a PGK-puromycin resistance cassette in WEC9.6 cells in which both Hist1h1c and Hist1he had already been modified. DNA from E7.5 embryos was examined PCR to confirm correct homologous recombination. Reverse phase HPLC of extracts from E10.5 embryos proved that no gene products were present. (J:83954)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:

2 assay results

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any H1f3 Mutation:	8 strains or lines available

Original:	J:83954 Fan Y, et al., H1 linker histones are essential for mouse development and affect nucleosome spacing in vivo. Mol Cell Biol. 2003 Jul;23(13):4559-72
All:	2 reference(s)