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Gene Expression Data
Assay Details
Assay
Reference: J:76230 Hajihosseini MK, et al., Expression patterns of fibroblast growth factors-18 and -20 in mouse embryos is suggestive of novel roles in calvarial and limb development. Mech Dev. 2002 Apr;113(1):79-83
Assay type: RNA in situ
MGI Accession ID: MGI:2665358
Gene symbol: Spp1
Gene name: secreted phosphoprotein 1
Probe: Spp1 probe2
Probe preparation: Antisense labelled with fluorescein RNA
Results
Specimen 2D: embryonic day 16.5 (more )
Note: Double labeled: orange - Spp1; purple - Fgf18 (assay MGI:2664829).
Structure Level Pattern Image Note
TS24: frontal bone Present 2D Expression directly abuts Fgf18 expression.
TS24: parietal bone Present 2D Expression directly abuts Fgf18 expression.
Specimen 2E: embryonic day 16.5 (more )
Note: Double labeled: orange - Spp1; purple - Fgf18 (assay MGI:2664829). Tissue was embedded in 2% agar and a 100-micron-thick vibratome section was cut.
Structure Level Pattern Image Note
TS24: frontal bone Present 2E Expression directly abuts Fgf18 expression.
TS24: parietal bone Present 2E Expression directly abuts Fgf18 expression.
Specimen 2F: embryonic day 16.5 (more )
Note: Double labeled: orange - Spp1; purple - Fgf18 (assay MGI:2664829). Tissue was embedded in 2% agar and a 100-micron-thick vibratome section was cut.
Structure Level Pattern Image Note
TS24: frontal bone Present 2F Expression directly abuts Fgf18 expression.
TS24: parietal bone Present 2F Expression directly abuts Fgf18 expression.
Specimen 2I: embryonic day 17.5 (more )
Note: Double labeled: orange - Spp1; purple - Fgf20 (assay MGI:2665356). Tissue was embedded in 2% agar and a 100-micron-thick vibratome section was cut.
Structure Level Pattern Image Note
TS25: frontal bone Present 2I There is some overlap in expression with Fgf20 in the ventral parts.
TS25: parietal bone Present 2I There is some overlap in expression with Fgf20 in the ventral parts.
Specimen 2L: embryonic day 17.5 (more )
Note: Double labeled: orange - Spp1; purple - Fgf9 (assay MGI:2665487). Tissue was embedded in 2% agar and a 100-micron-thick vibratome section was cut.
Structure Level Pattern Image Note
TS25: inter-parietal bone Present 2L There is no overlap with Fgf9 expression.
TS25: parietal bone Present 2L There is no overlap with Fgf9 expression.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory