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Gene Expression Data
Assay Details
Assay
Reference: J:42864 Dagenais A, et al., The alpha subunit of the epithelial sodium channel in the mouse: developmental regulation of its expression. Pediatr Res. 1997 Sep;42(3):327-34
Assay type: Northern blot
MGI Accession ID: MGI:2664796
Gene symbol: Scnn1a
Gene name: sodium channel, nonvoltage-gated 1 alpha
Probe: alpha-mENaC cDNA1
Probe preparation: Double stranded labelled with P32 DNA
Visualized with: Phosphorimaging
Assay notes: The densitometric quantification of this data is shown in Fig. 5.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 3.5 kb Amount Genetic BackgroundMutant Allele(s)Sex Note
E16.5 E16.5 (a) TS24: lung Absent 10 µg; total RNA CD-1 Not Specified
E17.5 E17.5 (a) TS25: lung Present 10 µg; total RNA CD-1 Not Specified
E18.5 E18.5 (a) TS26: lung Present 10 µg; total RNA CD-1 Not Specified
E19 E19.0 (a) TS26: lung Present 10 µg; total RNA CD-1 Not Specified
NB P newborn TS27: lung Present (b) 10 µg; total RNA CD-1 Not Specified These animals were collected two hours after birth.
1 P1 TS27: lung Present 10 µg; total RNA CD-1 Not Specified
3 P3 TS27: lung Present 10 µg; total RNA CD-1 Not Specified
5 P5 TS28: lung Present 10 µg; total RNA CD-1 Not Specified
AD P adult TS28: lung Present 10 µg; total RNA CD-1 Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) At birth there is a 2.5 fold increase in expression levels relative to that seen at the surrounding ages.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory