Selenop Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:40484 Kasik JW, et al., Selenoprotein P expression in liver, uterus and placenta during late pregnancy. Placenta. 1995 Jan;16(1):67-74
Assay type:	Northern blot
MGI Accession ID:	MGI:2664708
Gene symbol:	Selenop
Gene name:	selenoprotein P

Probe:	Sepp1 cDNA1
Probe preparation:	Double stranded DNA
Visualized with:	Autoradiography
Assay notes:	Each RNA sample is from a minimum of three mice.

Results

Image: 2A

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	2 kb (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
Uterus -4	E16.0 (a)	TS28: uterus	Trace	10 µg; total RNA	CD-1		Female
Uterus -3	E17.0 (a)	TS28: uterus	Trace	10 µg; total RNA	CD-1		Female
Uterus -2	E18.0 (a)	TS28: uterus	Trace	10 µg; total RNA	CD-1		Female
Uterus -1	E19.0 (a)	TS28: uterus	Trace	10 µg; total RNA	CD-1		Female
Uterus B	P0	TS27: uterus	Trace	10 µg; total RNA	CD-1		Female
Placenta -8	E12.0 (a)	TS28: placenta	Present	10 µg; total RNA	CD-1		Female
Placenta -7	E13.0 (a)	TS28: placenta	Present	10 µg; total RNA	CD-1		Female
Placenta -6	E14.0 (a)	TS28: placenta	Present	10 µg; total RNA	CD-1		Female
Placenta -5	E15.0 (a)	TS28: placenta	Present	10 µg; total RNA	CD-1		Female
Placenta -4	E16.0 (a)	TS28: placenta	Present	10 µg; total RNA	CD-1		Female
Placenta -3	E17.0 (a)	TS28: placenta	Present	10 µg; total RNA	CD-1		Female
Placenta -2	E18.0 (a)	TS28: placenta	Present	10 µg; total RNA	CD-1		Female
Placenta -1	E19.0 (a)	TS28: placenta	Present (c)	10 µg; total RNA	CD-1		Female
Adult liver	P adult	TS28: liver	Strong	10 µg; total RNA	CD-1		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference. Embryonic days were assigned by a curator based on the authors' assertion that birth occurred at E20.
(b) Beginning four days before birth, levels of expression in the placenta increase, reaching maximum levels at term. Expression levels did not change in the uterus.
(c) The level of expression in the placenta at its maximum is of 25% of that found in adult liver.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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