Dio3 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:80434 Hernandez A, et al., The gene locus encoding iodothyronine deiodinase type 3 (Dio3) is imprinted in the fetus and expresses antisense transcripts. Endocrinology. 2002 Nov;143(11):4483-6
Assay type:	Northern blot
MGI Accession ID:	MGI:2664605
Gene symbol:	Dio3
Gene name:	deiodinase, iodothyronine type III

Probe:	Dio3 cDNA1
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Autoradiography

Results

Image: 2C

	Sample Information		Bands			Other Sample Information
Lane	Age	Structure	4 kb (b)	3.5 kb (c)	2.2 kb (d)	Amount	Genetic Background	Mutant Allele(s)	Sex
+/-Dio3 (Paternal)	E15.0 (a)	TS23: embryo	Present	Present	Absent	2 µg; poly-A+ RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Dio3^tm1Stg/Dio3⁺	Not Specified
+/-Dio3 (Maternal)	E15.0 (a)	TS23: embryo	Absent	Absent	Present	2 µg; poly-A+ RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Dio3^tm1Stg/Dio3⁺	Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Corresponds to mutated allele; contains neomycin cassette. Results from use of native polyadenylation signaling sequence in the Dio3 gene
(c) Corresponds to mutated allele; contains neomycin cassette. Results from use of polyadenylation signaling sequence in the neomycin cassette.
(d) Corresponds to wild type allele.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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