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Gene Expression Data
Assay Details
Assay
Reference: J:58779 Nosaka K, et al., Molecular cloning and expression of a mouse thiamin pyrophosphokinase cDNA. J Biol Chem. 1999 Nov 26;274(48):34129-33
Assay type: Northern blot
MGI Accession ID: MGI:2663883
Gene symbol: Tpk1
Gene name: thiamine pyrophosphokinase
Probe: mTPK1 probe1
Probe preparation: labelled with P32 DNA
Visualized with: Autoradiography
Assay notes: Authors used two methods of signal detection, autoradiography and phophorimaging; only the autoradiograph is presented in the figure.
Results
Image: 5A ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 2.6 kb Amount Genetic BackgroundMutant Allele(s)Sex
heart P adult TS28: heart Trace (a) 2 µg; poly-A+ RNA Not Specified Not Specified
brain P adult TS28: brain Trace (a) 2 µg; poly-A+ RNA Not Specified Not Specified
spleen P adult TS28: spleen Not Specified 2 µg; poly-A+ RNA Not Specified Not Specified
lung P adult TS28: lung Not Specified 2 µg; poly-A+ RNA Not Specified Not Specified
liver P adult TS28: liver Present 2 µg; poly-A+ RNA Not Specified Not Specified
skeletal muscle P adult TS28: skeletal muscle Not Specified 2 µg; poly-A+ RNA Not Specified Not Specified
kidney P adult TS28: metanephros Present 2 µg; poly-A+ RNA Not Specified Not Specified
testis P adult TS28: testis Trace (a) 2 µg; poly-A+ RNA Not Specified Male
Notes:
(a) A faint band was detected after phosphorimaging.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory