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Gene Expression Data
Assay Details
Assay
Reference: J:64422 Wang LH, et al., A PDZ protein regulates the distribution of the transmembrane semaphorin, M-SemF. J Biol Chem. 1999 May 14;274(20):14137-46
Assay type: Northern blot
MGI Accession ID: MGI:2663699
Gene symbol: Sema5a
Gene name: sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
Probe: M-SemF cDNA1
Probe preparation: labelled with P32 DNA
Visualized with: Autoradiography
Results
Image: 1A ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 4.5 kb Amount Genetic BackgroundMutant Allele(s)Sex
E8 E8.0 (a) TS12: embryo Absent 20 µg; total RNA Not Specified Not Specified
E12 E12.0 (a) TS20: embryo Present 20 µg; total RNA Not Specified Not Specified
E15 E15.0 (a) TS23: head Present 20 µg; total RNA Not Specified Not Specified
E20 E20.0 (a) TS26: brain Present 20 µg; total RNA Not Specified Not Specified
P1 P1 TS27: brain Present 20 µg; total RNA Not Specified Not Specified
Adult S P adult TS28: spinal cord Present (b) 20 µg; total RNA Not Specified Not Specified
Adult B P adult TS28: forebrain Present (b) 20 µg; total RNA Not Specified Not Specified
Adult H P adult TS28: heart Absent 20 µg; total RNA Not Specified Not Specified
Adult K P adult TS28: metanephros Absent 20 µg; total RNA Not Specified Not Specified
Adult V P adult TS28: liver Absent 20 µg; total RNA Not Specified Not Specified
Adult L P adult TS28: lung Weak 20 µg; total RNA Not Specified Not Specified
Adult M P adult TS28: skeletal muscle Absent 20 µg; total RNA Not Specified Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Authors state that expression was detected primarily in the nervous system.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/16/2024
MGI 6.23
The Jackson Laboratory