Gene Expression Data
Assay Details
Assay
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Reference:
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J:39896
Abbaszade IG, et al., Isolation of a new mouse 3beta-hydroxysteroid dehydrogenase isoform, 3beta-HSD VI, expressed during early pregnancy. Endocrinology. 1997 Apr;138(4):1392-9
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:2663611
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Gene symbol:
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Hsd3b6
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Gene name:
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hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 6
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Probe:
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3-beta-HSD P1
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Probe preparation:
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DNA
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Assay notes:
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Identification of RT-PCR products was established by digestion with specific restriction enzymes. Authors state that fragment size is diagnostic for each of the Hsd3b gene products. Enzyme used in this assay digests both Hsd3b4 and Hsd3b5 gene products.
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Results
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Image: 7_VI
Control |
Control |
Control |
Control |
Control |
P adult
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TS28: testis
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Present
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0.1 µg; total RNA |
either: C57BL/6J or (involves: C57BL * BALB/c) or (involves: C57BL * SJL) |
| Male |
Control |
Control |
Control |
Control |
Control |
P adult
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TS28: ovary
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Absent
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0.1 µg; total RNA |
either: C57BL/6J or (involves: C57BL * BALB/c) or (involves: C57BL * SJL) |
| Female |
Control |
Control |
Control |
Control |
Control |
P adult
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TS28: adrenal gland
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Absent
|
0.1 µg; total RNA |
either: C57BL/6J or (involves: C57BL * BALB/c) or (involves: C57BL * SJL) |
| Male |
Control |
Control |
Control |
Control |
Control |
P adult
|
TS28: adrenal gland
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Absent
|
0.1 µg; total RNA |
either: C57BL/6J or (involves: C57BL * BALB/c) or (involves: C57BL * SJL) |
| Female |
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