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Gene Expression Data
Assay Details
Assay
Reference: J:39896 Abbaszade IG, et al., Isolation of a new mouse 3beta-hydroxysteroid dehydrogenase isoform, 3beta-HSD VI, expressed during early pregnancy. Endocrinology. 1997 Apr;138(4):1392-9
Assay type: RT-PCR
MGI Accession ID: MGI:2663610
Gene symbol: Hsd3b5
Gene name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 5
Probe: 3-beta-HSD P1
Probe preparation: DNA
Assay notes: Identification of RT-PCR products was established by digestion with specific restriction enzymes. Authors state that fragment size is diagnostic for each of the Hsd3b gene products. Enzyme used in this assay digests both Hsd3b4 and Hsd3b5 gene products.
Results Image: 7_IV
 Sample Information Bands Other Sample Information
Lane AgeStructure 629 bp Amount Genetic BackgroundMutant Allele(s)Sex
gonads male U Control
gonads male I Control
gonads male II Control
gonads male III Control
gonads male IV P adult TS28: testis Absent 0.1 µg; total RNA either: C57BL/6J or (involves: C57BL * BALB/c) or (involves: C57BL * SJL) Male
gonads male VI Control
gonads female U Control
gonads female I Control
gonads female II Control
gonads female III Control
gonads female IV P adult TS28: ovary Absent 0.1 µg; total RNA either: C57BL/6J or (involves: C57BL * BALB/c) or (involves: C57BL * SJL) Female
gonads female VI Control
adrenal glands male U Control
adrenal glands male I Control
adrenal glands male II Control
adrenal glands male III Control
adrenal glands male IV P adult TS28: adrenal gland Absent 0.1 µg; total RNA either: C57BL/6J or (involves: C57BL * BALB/c) or (involves: C57BL * SJL) Male
adrenal glands male VI Control
adrenal glands female U Control
adrenal glands female I Control
adrenal glands female II Control
adrenal glands female III Control
adrenal glands female IV P adult TS28: adrenal gland Absent 0.1 µg; total RNA either: C57BL/6J or (involves: C57BL * BALB/c) or (involves: C57BL * SJL) Female
adrenal glands female VI Control
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
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