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Gene Expression Data
Assay Details
Assay
Reference: J:53886 Kim E, et al., Disruption of the mouse Rce1 gene results in defective Ras processing and mislocalization of Ras within cells. J Biol Chem. 1999 Mar 26;274(13):8383-90
Assay type: Western blot
MGI Accession ID: MGI:2663519
Gene symbol: Hras
Gene name: Harvey rat sarcoma virus oncogene
Antibody: Anti-pan-Ras
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: The protein sample was immunoprecipitated with an antibody (MGI:2663500) that recognizes Hras1 (MGI:96224), Nras (MGI:97376) and Kras2 (MGI:96680). The antibody used in this Western assay recognizes the same three proteins.
Results
Image: 5D ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (b) Genetic BackgroundMutant Allele(s)Sex
Embryos Rce1-/- E14.5 (a) TS22: embryo Present involves: 129/Sv * C57BL/6 Rce1tm1Kim/Rce1tm1KimNot Specified
Embryos Rce1+/+ E14.5 (a) TS22: embryo Present involves: 129 * C57BL/6 Not Specified
Cells Rce1-/- In vitro data: no data stored
Cells Rce1+/+ In vitro data: no data stored
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Due to a processing defect the protein mobility was slightly retarded in the -/- lane.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
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