Nras Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:53886 Kim E, et al., Disruption of the mouse Rce1 gene results in defective Ras processing and mislocalization of Ras within cells. J Biol Chem. 1999 Mar 26;274(13):8383-90
Assay type:	Western blot
MGI Accession ID:	MGI:2663513
Gene symbol:	Nras
Gene name:	neuroblastoma ras oncogene

Antibody:	Anti-pan-Ras
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	The antibody used in this assay also recognizes Hras1 (MGI:96224) and Kras2 (MGI:96680).

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (b)	Genetic Background	Mutant Allele(s)	Sex
Rce1 +/+	E14.5 (a)	TS22: embryo	Present	involves: 129 * C57BL/6		Not Specified
Rce1 -/-	E14.5 (a)	TS22: embryo	Present	involves: 129/Sv * C57BL/6	Rce1^tm1Kim/Rce1^tm1Kim	Not Specified
Mixture	Control

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Due to a processing defect the protein mobility was slightly retarded in the -/- lane. In the mixture lane, the processed and unprocessed forms can be seen as a doublet.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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