Hsd3b6 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:39896 Abbaszade IG, et al., Isolation of a new mouse 3beta-hydroxysteroid dehydrogenase isoform, 3beta-HSD VI, expressed during early pregnancy. Endocrinology. 1997 Apr;138(4):1392-9
Assay type:	RT-PCR
MGI Accession ID:	MGI:2663463
Gene symbol:	Hsd3b6
Gene name:	hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 6

Probe:	3-beta-HSD P1
Probe preparation:	DNA
Assay notes:	Identification of RT-PCR products was established by digestion with specific restriction enzymes. Authors state that fragment size is diagnostic for each of the Hsd3b gene products.

Results

Image: 3_VI

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	629 bp	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
A 7d U	Control
A 7d I	Control
A 7d II	Control
A 7d III	Control
A 7d IV	Control
A 7d VI	E7.0 (a)	TS10: conceptus	Present	0.002 µg; total RNA	Not Specified		Not Specified	RNA from ClonTech. Authors state that samples include yolk sac and embryonic membranes.
B 9.5d U	Control
B 9.5d I	Control
B 9.5d II	Control
B 9.5d III	Control
B 9.5d IV	Control
B 9.5d VI	E9.5	TS15: conceptus	Present	0.2 µg; total RNA	C57BL/6J		Not Specified	Samples include yolk sac and embryonic membranes.
C 10.5d U	Control
C 10.5d I	Control
C 10.5d II	Control
C 10.5d III	Control
C 10.5d IV	Control
C 10.5d VI	E10.5	TS17: embryo	Present (b)	0.2 µg; total RNA	C57BL/6J		Not Specified	Represents two different embryos from the same litter of a C57BL/6J mother. Embryos were separated from yolk sac and placenta.
D 10.5d U	Control
D 10.5d I	Control
D 10.5d II	Control
D 10.5d III	Control
D 10.5d IV	Control
D 10.5d VI	E10.5	TS17: embryo	Present	0.2 µg; total RNA	involves: C57BL/6J * SJL/J		Not Specified	Representative of one of several embryos examined from a F2 litter of C57BL/6JxSJL/J parents. Embryos were separated from yolk sac and placenta.

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Authors report that expression was observed in embryos of either sex.

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