Hsd3b4 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:39896 Abbaszade IG, et al., Isolation of a new mouse 3beta-hydroxysteroid dehydrogenase isoform, 3beta-HSD VI, expressed during early pregnancy. Endocrinology. 1997 Apr;138(4):1392-9
Assay type:	RT-PCR
MGI Accession ID:	MGI:2663460
Gene symbol:	Hsd3b4
Gene name:	hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 4

Probe:	3-beta-HSD P1
Probe preparation:	DNA
Assay notes:	Identification of RT-PCR products was established by digestion with specific restriction enzymes. Authors state that fragment size is diagnostic for each of the Hsd3b gene products. Enzyme used in this assay digests both Hsd3b4 and Hsd3b5 gene products.

Results

Image: 3_IV

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	629 bp	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
A 7d U	Control
A 7d I	Control
A 7d II	Control
A 7d III	Control
A 7d IV	E7.0 (a)	TS10: conceptus	Absent	0.002 µg; total RNA	Not Specified		Not Specified	RNA from ClonTech. Authors state that samples include yolk sac and embryonic membranes.
A 7d VI	Control
B 9.5d U	Control
B 9.5d I	Control
B 9.5d II	Control
B 9.5d III	Control
B 9.5d IV	E9.5	TS15: conceptus	Absent	0.2 µg; total RNA	C57BL/6J		Not Specified	Samples include yolk sac and embryonic membranes.
B 9.5d VI	Control
C 10.5d U	Control
C 10.5d I	Control
C 10.5d II	Control
C 10.5d III	Control
C 10.5d IV	E10.5	TS17: embryo	Absent	0.2 µg; total RNA	C57BL/6J		Not Specified	Represents two different embryos from the same litter of a C57BL/6J mother. Embryos were separated from yolk sac and placenta.
C 10.5d VI	Control
D 10.5d U	Control
D 10.5d I	Control
D 10.5d II	Control
D 10.5d III	Control
D 10.5d IV	E10.5	TS17: embryo	Absent	0.2 µg; total RNA	involves: C57BL/6J * SJL/J		Not Specified	Representative of one of several embryos examined from a F2 litter of C57BL/6JxSJL/J parents. Embryos were separated from yolk sac and placenta.
D 10.5d VI	Control

Notes:
(a) Age of embryo at noon of plug day not specified in reference.

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