Gene Expression Data
Assay Details
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Assay
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Reference:
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J:39896
Abbaszade IG, et al., Isolation of a new mouse 3beta-hydroxysteroid dehydrogenase isoform, 3beta-HSD VI, expressed during early pregnancy. Endocrinology. 1997 Apr;138(4):1392-9
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:2663459
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Gene symbol:
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Hsd3b3
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Gene name:
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hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 3
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Probe:
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3-beta-HSD P1
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Probe preparation:
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DNA
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Assay notes:
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Identification of RT-PCR products was established by digestion with specific restriction enzymes. Authors state that fragment size is diagnostic for each of the Hsd3b gene products.
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Results
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Image: 3_III
| Control |
| Control |
| Control |
| E7.0
(a)
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TS10: conceptus
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Absent
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0.002 µg; total RNA |
Not Specified |
| Not Specified |
RNA from ClonTech. Authors state that samples include yolk sac and embryonic membranes.
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| Control |
| Control |
| Control |
| Control |
| Control |
| E9.5
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TS15: conceptus
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Absent
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0.2 µg; total RNA |
C57BL/6J |
| Not Specified |
Samples include yolk sac and embryonic membranes.
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| Control |
| Control |
| Control |
| Control |
| Control |
| E10.5
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TS17: embryo
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Absent
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0.2 µg; total RNA |
C57BL/6J |
| Not Specified |
Represents two different embryos from the same litter of a C57BL/6J mother. Embryos were separated from yolk sac and placenta.
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| Control |
| Control |
| Control |
| Control |
| Control |
| E10.5
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TS17: embryo
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Absent
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0.2 µg; total RNA |
C57BL/6J |
| Not Specified |
Representative of one of several embryos examined from a F2 litter of C57BL/6JxSJL/J parents. Embryos were separated from yolk sac and placenta.
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| Control |
| Control |
Notes:
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Analysis Tools