Hsd3b1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:39896 Abbaszade IG, et al., Isolation of a new mouse 3beta-hydroxysteroid dehydrogenase isoform, 3beta-HSD VI, expressed during early pregnancy. Endocrinology. 1997 Apr;138(4):1392-9
Assay type:	RT-PCR
MGI Accession ID:	MGI:2663419
Gene symbol:	Hsd3b1
Gene name:	hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1

Probe:	3-beta-HSD P1
Probe preparation:	DNA
Assay notes:	Identification of RT-PCR products was established by digestion with specific restriction enzymes. Authors state that fragment size is diagnostic for each of the Hsd3b gene products.

Results

Image: 7_I

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	629 bp	Amount	Genetic Background	Mutant Allele(s)	Sex
gonads male U	Control
gonads male I	P adult	TS28: testis	Present	0.1 µg; total RNA	either: C57BL/6J or (involves: C57BL * BALB/c) or (involves: C57BL * SJL)		Male
gonads male II	Control
gonads male III	Control
gonads male IV	Control
gonads male VI	Control
gonads female U	Control
gonads female I	P adult	TS28: ovary	Present	0.1 µg; total RNA	either: C57BL/6J or (involves: C57BL * BALB/c) or (involves: C57BL * SJL)		Female
gonads female II	Control
gonads female III	Control
gonads female IV	Control
gonads female VI	Control
adrenal glands male U	Control
adrenal glands male I	P adult	TS28: adrenal gland	Present	0.1 µg; total RNA	either: C57BL/6J or (involves: C57BL * BALB/c) or (involves: C57BL * SJL)		Male
adrenal glands male II	Control
adrenal glands male III	Control
adrenal glands male IV	Control
adrenal glands male VI	Control
adrenal glands female U	Control
adrenal glands female I	P adult	TS28: adrenal gland	Present	0.1 µg; total RNA	either: C57BL/6J or (involves: C57BL * BALB/c) or (involves: C57BL * SJL)		Female
adrenal glands female II	Control
adrenal glands female III	Control
adrenal glands female IV	Control
adrenal glands female VI	Control

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/23/2024 MGI 6.23