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Gene Expression Data
Assay Details
Assay
Reference: J:39896 Abbaszade IG, et al., Isolation of a new mouse 3beta-hydroxysteroid dehydrogenase isoform, 3beta-HSD VI, expressed during early pregnancy. Endocrinology. 1997 Apr;138(4):1392-9
Assay type: RT-PCR
MGI Accession ID: MGI:2663417
Gene symbol: Hsd3b1
Gene name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
Probe: 3-beta-HSD P1
Probe preparation: DNA
Assay notes: Identification of RT-PCR products was established by digestion with specific restriction enzymes. Authors state that fragment size is diagnostic for each of the Hsd3b gene products. RT-PCR products were subsequently analyzed by Southern blot hybridization using a radiolabelled cDNA probe.
Results Image: 5_I
 Sample Information Bands Other Sample Information
Lane AgeStructure 629 bp Amount Genetic BackgroundMutant Allele(s)Sex Note
A U Control
A I E9.5 TS15: embryo Weak (a) 0.5 µg; total RNA either: (involves: C57BL * BALB/c) or (involves: C57BL * SJL) Not Specified
A II Control
A III Control
A IV Control
A VI Control
B U Control
B I P adult TS28: uterus Weak (a) 0.7 µg; total RNA either: (involves: C57BL * BALB/c) or (involves: C57BL * SJL) Female uterus at embryonic day 7.5
B II Control
B III Control
B IV Control
B VI Control
Notes:
(a) Authors report minimal expression.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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