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Gene Expression Data
Assay Details
Assay
Reference: J:28733 Kim KK, et al., Tumor suppressor gene expression during normal and pathologic myocardial growth. J Biol Chem. 1994 Sep 9;269(36):22607-13
Assay type: Western blot
MGI Accession ID: MGI:2661076
Gene symbol: Rb1
Gene name: RB transcriptional corepressor 1
Antibody: anti-RB (clone G3-245)
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: The embryonic liver protein was titrated from 50 ug to 0 ug, but the weight of protein in each sample was adjusted to 50 ug with adult liver lysate (expression is absent in the adult tissue). A lighter exposure of the blot is in Fig. 4A.
Results
Image: 4B ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (b) Genetic BackgroundMutant Allele(s)Sex
50 ug E15.0 (a) TS23: liver Present Not Specified Not Specified
10 ug E15.0 (a) TS23: liver Not Specified Not Specified Not Specified
2 ug E15.0 (a) TS23: liver Not Specified Not Specified Not Specified
1 ug E15.0 (a) TS23: liver Not Specified Not Specified Not Specified
0 ug P adult TS28: liver Absent Not Specified Not Specified
EMB E15.0 (a) TS23: heart Present Not Specified Not Specified
NEO P1 TS27: heart Present Not Specified Not Specified
AD P adult TS28: heart Weak Not Specified Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Embryonic and neonatal heart expression levels were 20-fold lower than embryonic liver.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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