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Gene Expression Data
Assay Details
Assay
Reference: J:31819 Chaudhari N, et al., mRNA for cardiac calcium channel is expressed during development of skeletal muscle. Dev Biol. 1993 Feb;155(2):507-15
Assay type: Northern blot
MGI Accession ID: MGI:2660783
Gene symbol: Cacna1s
Gene name: calcium channel, voltage-dependent, L type, alpha 1S subunit
Probe: S16
Probe preparation: Double stranded DNA
Visualized with: Autoradiography
Assay notes: Authors state that expression of the 6.6kb transcript increases considerably during normal fetal development and suggest that the 6.6 kb transcript is the mature RNA. Fig. 1.B shows the E17m lanes of both the cardiac and skeletal blots fdrom Fig. 1.A side-by-side.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 10 kb 7.6 kb 6.6 kb Amount Genetic BackgroundMutant Allele(s)Sex
F14 E14.0 (a) TS22: skeletal muscle Present Present Present 10 µg; poly-A+ RNA Not Specified Not Specified
F16+ E16.0 (a) TS24: skeletal muscle Present Present Present 10 µg; poly-A+ RNA Not Specified Not Specified
F16m E16.0 (a) TS24: skeletal muscle Present Present Present 10 µg; poly-A+ RNA Not Specified Cacna1smdg/Cacna1smdgNot Specified
F17+ E17.0 (a) TS25: skeletal muscle Present Present Present 10 µg; poly-A+ RNA Not Specified Not Specified
F17m E17.0 (a) TS25: skeletal muscle Present Present Present 10 µg; poly-A+ RNA Not Specified Cacna1smdg/Cacna1smdgNot Specified
F19+ E19.0 (a) TS26: skeletal muscle Present Present Present 10 µg; poly-A+ RNA Not Specified Not Specified
F19m E19.0 (a) TS26: skeletal muscle Present Present Present 10 µg; poly-A+ RNA Not Specified Cacna1smdg/Cacna1smdgNot Specified
H P adult TS28: heart Present Present Present 10 µg; poly-A+ RNA Not Specified Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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