Gene Expression Data
Assay Details
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Assay
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Reference:
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J:16427
Desbois C, et al., The mouse osteocalcin gene cluster contains three genes with two separate spatial and temporal patterns of expression. J Biol Chem. 1994 Jan 14;269(2):1183-90
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:2660760
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Gene symbol:
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Bglap
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Gene name:
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bone gamma carboxyglutamate protein
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Probe:
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Osteocalcin-pA, Osteocalcin-pB
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Probe preparation:
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DNA
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Assay notes:
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The RT-PCR reaction was electrophoresed and Southern blotted using an oligonucleotide probe. The primers potentially amplified products from three genes, but the Southern probe specifically recognized Bglap1 and Bglap2.
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Results
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| Control |
| Control |
| E9.5
(a)
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TS15: embryo
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Absent
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1 µg; total RNA |
Not Specified |
| Not Specified |
| E10.5
(a)
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TS17: embryo
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Absent
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1 µg; total RNA |
Not Specified |
| Not Specified |
| E11.5
(a)
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TS19: embryo
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Absent
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1 µg; total RNA |
Not Specified |
| Not Specified |
| E12.5
(a)
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TS20: embryo
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Absent
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1 µg; total RNA |
Not Specified |
| Not Specified |
| E13.5
(a)
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TS21: embryo
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Absent
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1 µg; total RNA |
Not Specified |
| Not Specified |
| E14.5
(a)
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TS22: embryo
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Absent
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1 µg; total RNA |
Not Specified |
| Not Specified |
| E15.5
(a)
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TS23: embryo
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Present
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1 µg; total RNA |
Not Specified |
| Not Specified |
| E16.5
(a)
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TS24: embryo
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Present
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1 µg; total RNA |
Not Specified |
| Not Specified |
| E17.5
(a)
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TS25: embryo
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Present
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1 µg; total RNA |
Not Specified |
| Not Specified |
| Control |
| Control |
| Control |
Notes:
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Analysis Tools