Zfp57 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:17110 Okazaki S, et al., A novel nuclear protein with zinc fingers down-regulated during early mammalian cell differentiation. J Biol Chem. 1994 Mar 4;269(9):6900-7
Assay type:	Northern blot
MGI Accession ID:	MGI:2660689
Gene symbol:	Zfp57
Gene name:	zinc finger protein 57

Probe:	Zfp57 probe b
Probe preparation:	labelled with P32 DNA
Visualized with:	Autoradiography

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
Day 8	E8.0 (a)	TS12: embryo	Not Specified	10 µg; total RNA	C3H/He		Male
Day 9	E9.0 (a)	TS14: embryo	Not Specified	10 µg; total RNA	C3H/He		Male
Day 10	E10.0 (a)	TS16: embryo	Not Specified	10 µg; total RNA	C3H/He		Male
Day 11	E11.0 (a)	TS18: embryo	Weak	10 µg; total RNA	C3H/He		Male
Day 12	E12.0 (a)	TS20: embryo	Weak	10 µg; total RNA	C3H/He		Male
Day 13	E13.0 (a)	TS21: embryo	Present	10 µg; total RNA	C3H/He		Male
Day 14	E14.0 (a)	TS22: embryo	Weak	10 µg; total RNA	C3H/He		Male
Day 15	E15.0 (a)	TS23: embryo	Weak	10 µg; total RNA	C3H/He		Male
Day 16	E16.0 (a)	TS24: embryo	Weak	10 µg; total RNA	C3H/He		Male
Day 17	E17.0 (a)	TS25: embryo	Weak	10 µg; total RNA	C3H/He		Male
Day 18	E18.0 (a)	TS26: embryo	Weak	10 µg; total RNA	C3H/He		Male
Day 19	E19.0 (a)	TS26: embryo	Weak	10 µg; total RNA	C3H/He		Male

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Authors state that there are two alternative splice forms (1.8kb and 3.2kb) but do not specify which one or both is expressed in embryos. Expression increased from E11-E13 but was low in subsequent days.

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