Efs Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:30264 Ishino M, et al., Molecular cloning of a cDNA encoding a phosphoprotein, Efs, which contains a Src homology 3 domain and associates with Fyn. Oncogene. 1995 Dec 7;11(11):2331-8
Assay type:	Northern blot
MGI Accession ID:	MGI:2659135
Gene symbol:	Efs
Gene name:	embryonal Fyn-associated substrate

Probe:	Efs cDNA3
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Autoradiography
Assay notes:	The probe used in this assay was against the amino terminal region.

Results

Image: 3_Efs

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
liver	P adult	TS28: liver	Absent	10 µg; total RNA	BALB/c		Female
kidney	P adult	TS28: metanephros	Not Specified	10 µg; total RNA	BALB/c		Female
spleen	P adult	TS28: spleen	Not Specified	10 µg; total RNA	BALB/c		Female
lung	P adult	TS28: lung	Weak	10 µg; total RNA	BALB/c		Female
intestine	P adult	TS28: intestine	Absent	10 µg; total RNA	BALB/c		Female
skeletal muscle	P adult	TS28: skeletal muscle	Present (a)	10 µg; total RNA	BALB/c		Female
brain	P adult	TS28: brain	Present (b)	10 µg; total RNA	BALB/c		Female
brainstem	P adult	TS28: brainstem	Present	10 µg; total RNA	BALB/c		Female
placenta	E17.0	TS28: placenta	Strong (a)	10 µg; total RNA	BALB/c		Female
embryo	E17.0 (c)	TS25: embryo	Strong (d)	10 µg; total RNA	BALB/c		Not Specified
PCC4	In vitro data: no data stored

Notes:
(a) The predominant transcript was 2.7kb.
(b) The predominant transcript was 3.7kb.
(c) Age of embryo at noon of plug day not specified in reference.
(d) The embryo contained 3.7 and 2.7kb transcripts.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 05/14/2024 MGI 6.23