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Gene Expression Data
Assay Details
Assay
Reference: J:50953 Jiang S, et al., Strain-dependent developmental relaxation of imprinting of an endogenous mouse gene, Kvlqt1. Genomics. 1998 Nov 1;53(3):395-9
Assay type: RT-PCR
MGI Accession ID: MGI:2659117
Gene symbol: Kcnq1
Gene name: potassium voltage-gated channel, subfamily Q, member 1
Probe: U1, L1
Assay notes: After RT-PCR analyses, the bands were extracted and digested with BsmFI which cleaves the CAST/Ei allele into a 140 and 97 bp fragments. This assay indicates complete genomic imprinting.
Results Image: 4a
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex Note
Control Undigested Control
Control Digested Control
Paternal Allele P adult TS28: mouse Present Absent 1 µg; total RNA 129S/SvEv Male Specific tissues were not detailed.
Maternal Allele P adult TS28: mouse Absent Present 1 µg; total RNA CAST/EiJ Female Specific tissues were not detailed.
F1 6.5e E6.5 (c) TS9: embryo Absent Present 1 µg; total RNA involves: 129S/SvEv * CAST/Ei Not Specified
Notes:
(a) This band represents the 129S/SvEv allele.
(b) This band represents the Cast/Ei allele.
(c) Age of embryo at noon of plug day not specified in reference.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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