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Gene Expression Data
Assay Details
Assay
Reference: J:30129 Nakamura M, et al., Identification of two isoforms of mouse neuropeptide Y-Y1 receptor generated by alternative splicing. Isolation, genomic structure, and functional expression of the receptors. J Biol Chem. 1995 Dec 15;270(50):30102-10
Assay type: RT-PCR
MGI Accession ID: MGI:2659038
Gene symbol: Npy1r
Gene name: neuropeptide Y receptor Y1
Probe: SP2, bAP2
Probe preparation: labelled with P32
Assay notes: RT-PCR reactions were electrophoresed (Fig. 3b) then Southern blotted and probed with a 32P-labeled 170bp coding region fragment common to both splice variants (MGI:2659029).
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 309 bp Amount Genetic BackgroundMutant Allele(s)Sex
Lane 1 Lane not used in this assay
Lane 2 Lane not used in this assay
Lane 3 P adult TS28: bone marrow Present (a) Not Specified; poly-A+ RNA Not Specified Not Specified
Lane 4 Lane not used in this assay
Lane 5 Lane not used in this assay
Notes:
(a) This band was specifically amplified from the beta splice variant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory