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Gene Expression Data
Assay Details
Assay
Reference: J:26852 Sun J, et al., Gene structure, chromosomal localization, and expression of the murine homologue of human proteinase inhibitor 6 (PI-6) suggests divergence of PI-6 from the ovalbumin serpins. J Biol Chem. 1995 Jul 7;270(27):16089-96
Assay type: RT-PCR
MGI Accession ID: MGI:2658901
Gene symbol: Serpinb6a
Gene name: serine (or cysteine) peptidase inhibitor, clade B, member 6a
Probe: PB105, PB101
Probe preparation: labelled with P32
Assay notes: The RT-PCR was run on a gel (Fig. 7 top) then Southern blotted and probed with a 32P-labeled oligonucleotide from exon 4 (MGI:2658891).
Results
Image: 7_bottom ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
1+ E15.0 (a) TS23: yolk sac Present 10 µg; total RNA Not Specified Pooled
1- Control
2+ E15.0 (a) TS28: placenta Absent 10 µg; total RNA Not Specified Pooled
2- Control
3+ E15.0 (a) TS23: liver Weak (b) 10 µg; total RNA Not Specified Pooled
3- Control
4+ E15.0 (a) TS23: heart Weak (b) 10 µg; total RNA Not Specified Pooled
4- Control
5+ E15.0 (a) TS23: gut Not Specified 10 µg; total RNA Not Specified Pooled
5- Control
6+ E15.0 (a) TS23: metanephros Present 10 µg; total RNA Not Specified Pooled
6- Control
7+ E15.0 (a) TS23: lung Present 10 µg; total RNA Not Specified Pooled
7- Control
8+ E15.0 (a) TS23: thymus primordium Not Specified 10 µg; total RNA Not Specified Pooled
8- Control
9+ E15.0 (a) TS23: brain Weak 10 µg; total RNA Not Specified Pooled
9- Control
10+ E15.0 (a) TS23: limb musculature Weak (b) 10 µg; total RNA Not Specified Pooled
10- Control
11+ In vitro data: no data stored
11- Control
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression was weak or absent.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory