Ezh1 RNase protection Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:41121 Laible G, et al., Mammalian homologues of the Polycomb-group gene Enhancer of zeste mediate gene silencing in Drosophila heterochromatin and at S. cerevisiae telomeres. EMBO J. 1997 Jun 2;16(11):3219-32
Assay type:	RNase protection
MGI Accession ID:	MGI:2657036
Gene symbol:	Ezh1
Gene name:	enhancer of zeste 1 polycomb repressive complex 2 subunit

Probe:	Ezh1 cDNA4
Probe preparation:	Antisense labelled with P32 RNA

Results

Image: 3_(top panel)

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	320 bp	Amount	Genetic Background	Mutant Allele(s)	Sex
ES	In vitro data: no data stored
E8diff	In vitro data: no data stored
E9.5	E9.5 (a)	TS15: embryo	Present (b)	10 µg; total RNA	Not Specified		Not Specified
E13 FL	E13.0 (a)	TS21: liver	Present (b)	10 µg; total RNA	Not Specified		Not Specified
E13 BR	E13.0 (a)	TS21: brain	Present (b)	10 µg; total RNA	Not Specified		Not Specified
E13 SM	E13.0 (a)	TS21: skeletal muscle	Present (b)	10 µg; total RNA	Not Specified		Not Specified
E17 FL	E17.0 (a)	TS25: liver	Present (b)	10 µg; total RNA	Not Specified		Not Specified
E17 BR	E17.0 (a)	TS25: brain	Present (b)	10 µg; total RNA	Not Specified		Not Specified
E17 SM	E17.0 (a)	TS25: skeletal muscle	Present (b)	10 µg; total RNA	Not Specified		Not Specified
adult KI	P w 6-8	TS28: metanephros	Strong (c)	10 µg; total RNA	Not Specified		Not Specified
adult SP	P w 6-8	TS28: spleen	Weak (d)	10 µg; total RNA	Not Specified		Not Specified
adult LI	P w 6-8	TS28: liver	Weak (d)	10 µg; total RNA	Not Specified		Not Specified
adult TH	P w 6-8	TS28: thymus	Weak (d)	10 µg; total RNA	Not Specified		Not Specified
adult BR	P w 6-8	TS28: brain	Strong (c)	10 µg; total RNA	Not Specified		Not Specified
adult SM	P w 6-8	TS28: skeletal muscle	Strong (c)	10 µg; total RNA	Not Specified		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Detected ubiquitously during embryogenesis; gradually upregulated during development.
(c) Expression reaches 4- to 5-fold higher levels in adult kidney, brain and skeletal muscle.
(d) Low levels in liver, spleen and thymus.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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