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Gene Expression Data
Assay Details
Assay
Reference: J:33387 Guimaraes MJ, et al., Molecular cloning and characterization of lysosomal sialic acid O-acetylesterase. J Biol Chem. 1996 Jun 7;271(23):13697-705
Assay type: Northern blot
MGI Accession ID: MGI:2655632
Gene symbol: Siae
Gene name: sialic acid acetylesterase
Probe: clone 165
Probe preparation: Double stranded labelled with P32 DNA
Assay notes: Additional experiments have demonstrated that 2 messages are present. The 4.3kb message is of higher abundance than the 2.7kb message and there is no alteration in the relative proportions of the 2 messages in any of the tissues analyzed (not shown). Only the 4.3kb message is shown in this assay.
Results
Image: 6A top ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 4.3 kb (b) Amount Genetic BackgroundMutant Allele(s)Sex
Y.S. E11.5 (a) TS19: yolk sac Strong 20 µg; total RNA Not Specified Not Specified
AGM E11.5 (a) TS19: aorta-gonad-mesonephros Strong 20 µg; total RNA Not Specified Not Specified
F.L. E11.5 (a) TS19: liver Strong 20 µg; total RNA Not Specified Not Specified
H E11.5 (a) TS19: head Present 20 µg; total RNA Not Specified Not Specified
Y.S. E15.0 (a) TS23: yolk sac Present 20 µg; total RNA Not Specified Not Specified
F.L. E15.0 (a) TS23: liver Present 20 µg; total RNA Not Specified Not Specified
H E15.0 (a) TS23: head Present 20 µg; total RNA Not Specified Not Specified
Plac. E15.0 (a) TS28: placenta Present 20 µg; total RNA Not Specified Not Specified
B.M. P adult TS28: bone marrow Not Specified 20 µg; total RNA Not Specified Not Specified
Liver P adult TS28: liver Strong 20 µg; total RNA Not Specified Not Specified
Brain P adult TS28: brain Not Specified 20 µg; total RNA Not Specified Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) There was a decrease in expression in all tissues analyzed from E11.5 to E15, however in the adult, the liver was found to be the organ where the message is most abundant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory