Zfy1 RT-PCR Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:40018 Koopman P, et al., Zfy gene expression patterns are not compatible with a primary role in mouse sex determination. Nature. 1989 Dec 21-28;342(6252):940-2
Assay type:	RT-PCR
MGI Accession ID:	MGI:2655577
Gene symbol:	Zfy1
Gene name:	zinc finger protein 1, Y-linked

Probe:	Zfy1-pC, Zfy1-pD
Visualized with:	Ethidium bromide
Assay notes:	Nested primers were used for this assay. After amplification with the first primer set, the authors used another primer set (MGI:2655583). Littermates from 3 separate litters were analyzed. This gene produces alternate transcripts, as reflected in the three different band sizes.

Results

Image: 4 ©

	Sample Information		Bands			Other Sample Information
Lane	Age	Structure	572 bp	523 bp	477 bp	Amount	Genetic Background	Mutant Allele(s)	Sex
1 w m	E14.5	TS22: testis	Absent	Absent	Absent	Not Specified	involves: 101/H * C3H/HeH	Kit^W-e/Kit^W-e	Male
1 + m	E14.5	TS22: testis	Present	Present	Present	Not Specified	involves: 101/H * C3H/HeH		Male
1 + f	E14.5	TS22: ovary	Absent	Absent	Absent	Not Specified	involves: 101/H * C3H/HeH		Female
2 w m	E14.5	TS22: testis	Absent	Absent	Absent	Not Specified	involves: 101/H * C3H/HeH	Kit^W-e/Kit^W-e	Male
2 + m	E14.5	TS22: testis	Present	Present	Present	Not Specified	involves: 101/H * C3H/HeH		Male
2 + f	E14.5	TS22: ovary	Absent	Absent	Absent	Not Specified	involves: 101/H * C3H/HeH		Female
3 w m	E14.5	TS22: testis	Absent	Absent	Absent	Not Specified	involves: 101/H * C3H/HeH	Kit^W-e/Kit^W-e	Male
3 + m	E14.5	TS22: testis	Present	Present	Present	Not Specified	involves: 101/H * C3H/HeH		Male
No RNA	Control
Marker	Mol. Wt. Marker Lane

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