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Gene Expression Data
Assay Details
Assay
Reference: J:40018 Koopman P, et al., Zfy gene expression patterns are not compatible with a primary role in mouse sex determination. Nature. 1989 Dec 21-28;342(6252):940-2
Assay type: RT-PCR
MGI Accession ID: MGI:2655567
Gene symbol: Zfy2
Gene name: zinc finger protein 2, Y-linked
Probe: Zfy2-pA, Zfy2-pB
Visualized with: Ethidium bromide
Assay notes: Nested primers were used for this assay. After amplification with the first primer set, the authors used another primer set (MGI:2655583). The amplified products also underwent Southern blotting, as pictured in Fig. 2a4.
Results
Image: 2a 3 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
Testis 0.1 P adult TS28: testis Present 0.1 µg; total RNA Parkes Male
Testis 0.01 P adult TS28: testis Present 0.01 µg; total RNA Parkes Male
Ov. P adult TS28: ovary Absent 1 µg; total RNA Parkes Female
Li. P adult TS28: liver Absent 1 µg; total RNA Parkes Male
9.5 male E9.5 TS15: trunk Absent 1 µg; total RNA Parkes Male embryos posterior to the forelimb bud
9.5 female E9.5 TS15: trunk Absent 1 µg; total RNA Parkes Female embryos posterior to the forelimb bud
10.5 male E10.5 TS17: trunk Absent 1 µg; total RNA Parkes Male embryos between forelimb and hindlimb buds
10.5 female E10.5 TS17: trunk Absent 1 µg; total RNA Parkes Female embryos between forelimb and hindlimb buds
11.5 male E11.5 TS19: gonad Absent 1 µg; total RNA Parkes Male
11.5 female E11.5 TS19: gonad Absent 1 µg; total RNA Parkes Female
12.5 male E12.5 TS20: testis Absent 1 µg; total RNA Parkes Male
12.5 female E12.5 TS20: ovary Absent 1 µg; total RNA Parkes Female
13.5 male E13.5 TS21: testis Absent 1 µg; total RNA Parkes Male
13.5 female E13.5 TS21: ovary Absent 1 µg; total RNA Parkes Female
14.5 male E14.5 TS22: testis Absent 1 µg; total RNA Parkes Male
14.5 female E14.5 TS22: ovary Absent 1 µg; total RNA Parkes Female
ES male In vitro data: no data stored
ES female In vitro data: no data stored
No RNA Control
11.5 male He E11.5 TS19: head Absent 1 µg; total RNA Parkes Male
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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