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Gene Expression Data
Assay Details
Assay
Reference: J:36725 Kim KK, et al., Mouse RAD50 has limited epitopic homology to p53 and is expressed in the adult myocardium. J Biol Chem. 1996 Nov 15;271(46):29255-64
Assay type: Northern blot
MGI Accession ID: MGI:2655076
Gene symbol: Rad50
Gene name: RAD50 double strand break repair protein
Probe: Rad50 cDNA1
Probe preparation: Double stranded labelled with radioactivity DNA
Visualized with: Autoradiography
Assay notes: The probe was radiolabeled. Densitometry was used to determine the relative level (%) of the 5.3kb transcript in each tissue as compared to AT-2 cardiomyocytes on the same blot. The values were further normalized to controls to account for variations inloading.
Results
Image: 6C ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 5.3 kb (a) 3.1 kb 1.6 kb Amount Genetic BackgroundMutant Allele(s)Sex
AT-2 (100%) In vitro data: no data stored
BRAIN (9%) P adult TS28: brain Not Specified Not Specified Not Specified 10 µg; total RNA C3HeB/FeJ Not Specified
GUT (4%) P adult TS28: gut Not Specified Not Specified Not Specified 10 µg; total RNA C3HeB/FeJ Not Specified
HEART (23%) P adult TS28: heart Strong Present Not Specified 10 µg; total RNA C3HeB/FeJ Not Specified
KIDNEY (11%) P adult TS28: metanephros Not Specified Not Specified Not Specified 10 µg; total RNA C3HeB/FeJ Not Specified
LIVER (6%) P adult TS28: liver Not Specified Not Specified Not Specified 10 µg; total RNA C3HeB/FeJ Not Specified
LUNG (16%) P adult TS28: lung Strong Present Not Specified 10 µg; total RNA C3HeB/FeJ Not Specified
SK. MUS. (5%) P adult TS28: skeletal muscle Not Specified Not Specified Not Specified 10 µg; total RNA C3HeB/FeJ Not Specified
SKIN (6%) P adult TS28: skin Not Specified Not Specified Not Specified 10 µg; total RNA C3HeB/FeJ Not Specified
SPLEEN (6%) P adult TS28: spleen Not Specified Not Specified Not Specified 10 µg; total RNA C3HeB/FeJ Not Specified
AORTA (16%) P adult TS28: aorta Strong Not Specified Not Specified 10 µg; total RNA C3HeB/FeJ Not Specified
TESTES (94%) P adult TS28: testis Very strong Not Specified Present 10 µg; total RNA C3HeB/FeJ Male
UTERUS (0%) P adult TS28: uterus Not Specified Not Specified Not Specified 10 µg; total RNA C3HeB/FeJ Female
Notes:
(a) There was little or no 5.3kb transcript in most tissues.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory