Mas1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:21842 Villar AJ, et al., Parental imprinting of the Mas protooncogene in mouse. Nat Genet. 1994 Dec;8(4):373-9
Assay type:	RT-PCR
MGI Accession ID:	MGI:2654370
Gene symbol:	Mas1
Gene name:	MAS1 oncogene

Probe:	Mas-pA, Mas-pB
Visualized with:	Ethidium bromide
Assay notes:	The products of the RT-PCR reaction were subjected to restriction enzyme digestion to generate species specific RLFPs.

Results

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	Size Not Specified (b)	Size Not Specified (c)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
Head	E11.0 (a)	TS18: head	Absent	Present	Not Specified; total RNA	involves: C57BL/6J * M. spretus		Not Specified
Trunk	E11.0 (a)	TS18: trunk	Absent	Present	Not Specified; total RNA	involves: C57BL/6J * M. spretus		Not Specified
v. yolk sac	E11.0 (a)	TS18: yolk sac	Absent	Present	Not Specified; total RNA	involves: C57BL/6J * M. spretus		Not Specified	visceral yolk sac

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) This band reflects expression from spretus allele, which was maternally inherited.
(c) This band reflects expression from the B6 allele.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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