About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:21842 Villar AJ, et al., Parental imprinting of the Mas protooncogene in mouse. Nat Genet. 1994 Dec;8(4):373-9
Assay type: RNase protection
MGI Accession ID: MGI:2654350
Gene symbol: Mas1
Gene name: MAS1 oncogene
Probe: Mas cDNA2
Probe preparation: Antisense labelled with S35 RNA
Visualized with: Autoradiography
Assay notes: The upper band on the blot is the Gapdh internal control.
Results Image: 5
 Sample Information Bands Other Sample Information
Lane AgeStructure 183 bp Amount Genetic BackgroundMutant Allele(s)Sex
Gapdh Control
Mas Control
Adult brain P adult TS28: brain Absent 5 µg; poly-A+ RNA C57BL/6J Not Specified
Adult brain P adult TS28: brain Present 10 µg; poly-A+ RNA C57BL/6J Not Specified
11.0 d.g. Fetus E11.0 (a) TS18: embryo Absent 5 µg; poly-A+ RNA C57BL/6J Not Specified
11.0 d.g. Fetus E11.0 (a) TS18: embryo Absent 10 µg; poly-A+ RNA C57BL/6J Not Specified
11.0 d.g. Fetus E11.0 (a) TS18: embryo Present (b) 15 µg; poly-A+ RNA C57BL/6J Not Specified
12.5 d.g. Fetus E12.5 (a) TS20: embryo Absent 5 µg; poly-A+ RNA C57BL/6J Not Specified
12.5 d.g. Fetus E12.5 (a) TS20: embryo Absent 10 µg; poly-A+ RNA C57BL/6J Not Specified
12.5 d.g. Fetus E12.5 (a) TS20: embryo Present (c) 15 µg; poly-A+ RNA C57BL/6J Not Specified
B Lane not used in this assay
yeast RNA Control
yeast RNA Control
yeast RNA Control
yeast RNA Control
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression in the E11.0 embryo is 57% of that seen in the adult brain.
(c) Expression in the E12.5 embryo is 76% of that seen in the adult brain.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory