Dnmt3a RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:80002 Weisenberger DJ, et al., Identification and characterization of alternatively spliced variants of DNA methyltransferase 3a in mammalian cells. Gene. 2002 Sep 18;298(1):91-9
Assay type:	RT-PCR
MGI Accession ID:	MGI:2654158
Gene symbol:	Dnmt3a
Gene name:	DNA methyltransferase 3A

Probe:	Exon1-beta-, exon4
Probe preparation:	labelled with P32
Visualized with:	Phosphorimaging

Results

Image: 2B Dnmt3a-B

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
heart	P adult	TS28: heart	Weak (a)	Not Specified	Not Specified		Not Specified
brain	P adult	TS28: brain	Weak (a)	Not Specified	Not Specified		Not Specified
spleen	P adult	TS28: spleen	Weak (a)	Not Specified	Not Specified		Not Specified
lung	P adult	TS28: lung	Weak (a)	Not Specified	Not Specified		Not Specified
liver	P adult	TS28: liver	Weak (a)	Not Specified	Not Specified		Not Specified
sk. muscle	P adult	TS28: skeletal muscle	Weak (a)	Not Specified	Not Specified		Not Specified
kidney	P adult	TS28: metanephros	Weak (a)	Not Specified	Not Specified		Not Specified
testis	P adult	TS28: testis	Weak (a)	Not Specified	Not Specified		Male
7	E7.0 (b)	TS10: embryo	Weak (a)	Not Specified	Not Specified		Not Specified
11	E11.0 (b)	TS18: embryo	Weak (a)	Not Specified	Not Specified		Not Specified
15	E15.0 (b)	TS23: embryo	Weak (a)	Not Specified	Not Specified		Not Specified
17	E17.0 (b)	TS25: embryo	Weak (a)	Not Specified	Not Specified		Not Specified

Notes:
(a) These values were low compared to expression of the other splice variant described in assay MGI:2654157.
(b) Age of embryo at noon of plug day not specified in reference.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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