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Gene Expression Data
Assay Details
Assay
Reference: J:40649 Chatot CL, et al., Analysis of glutaminase activity and RNA expression in preimplantation mouse embryos. Mol Reprod Dev. 1997 Jul;47(3):248-54
Assay type: RT-PCR
MGI Accession ID: MGI:2654032
Gene symbol: Gls
Gene name: glutaminase
Probe: Gls-pA, Gls-pB
Probe preparation: Antisense labelled with P32 RNA
Visualized with: Autoradiography
Assay notes: The RT-PCR products were visualized by hybridization with a radioactive probe.
Results
Image: 2B ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 341 bp (a) Amount Genetic BackgroundMutant Allele(s)Sex Note
Lane 1 Control
Lane 2 E0.5 (b) TS1: 1-cell stage conceptus Present Not Specified involves: C57BL/6 * NSA * SJL Not Specified
Lane 3 E1.0 (b) TS2: 2-cell stage conceptus Present Not Specified involves: C57BL/6 * NSA * SJL Not Specified early two cell embryos
Lane 4 E2.5 (b) TS3: 8-cell stage conceptus Present Not Specified involves: C57BL/6 * NSA * SJL Not Specified
Lane 5 E3.5 (b) TS4: blastocyst-stage conceptus Present Not Specified involves: C57BL/6 * NSA * SJL Not Specified
Lane 6 E1.5 (b) TS2: 2-cell stage conceptus Present Not Specified involves: C57BL/6 * NSA * SJL Not Specified late two cell embryos
Notes:
(a) Expression levels decreased from the one cell stage to the early 2 cell stage before showing a sustained increase from the late 2 cell stage to the blastocyst stage (the period of peak expression); quantification is provided in Fig 3.
(b) Age assigned by curator based on morphological criteria supplied by authors.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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