About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:40649 Chatot CL, et al., Analysis of glutaminase activity and RNA expression in preimplantation mouse embryos. Mol Reprod Dev. 1997 Jul;47(3):248-54
Assay type: RT-PCR
MGI Accession ID: MGI:2654031
Gene symbol: Gls
Gene name: glutaminase
Probe: Gls-pA, Gls-pB
Probe preparation: Antisense labelled with P32 RNA
Visualized with: Autoradiography
Assay notes: The RT-PCR products were visualized by hybridization with a radioactive probe.
Results
Image: 2A ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 341 bp (a) Amount Genetic BackgroundMutant Allele(s)Sex Note
Lane 1 Control
Lane 2 P TS28: brain Present 2 µg involves: C57BL/6 * NSA * SJL Not Specified
Lane 3 E0.5 (b) TS1: 1-cell stage conceptus Present Not Specified involves: C57BL/6 * NSA * SJL Not Specified
Lane 4 E2.5 (b) TS3: 8-cell stage conceptus Present Not Specified involves: C57BL/6 * NSA * SJL Not Specified
Lane 5 E3.5 (b) TS4: blastocyst-stage conceptus Present Not Specified involves: C57BL/6 * NSA * SJL Not Specified
Lane 6 E1.0 (b) TS2: 2-cell stage conceptus Present Not Specified involves: C57BL/6 * NSA * SJL Not Specified early two cell embryos
Lane 7 E1.5 (b) TS2: 2-cell stage conceptus Present Not Specified involves: C57BL/6 * NSA * SJL Not Specified late two cell embryos
Notes:
(a) Expression levels decreased from the one cell stage to the early 2 cell stage before showing a sustained increase from the late 2 cell stage to the blastocyst stage (the period of peak expression); quantification is provided in Fig 3.
(b) Age assigned by curator based on morphological criteria supplied by authors.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/09/2024
MGI 6.23 		The Jackson Laboratory