Tg(Igh-Creb1S133A)1Lbox
Transgene Detail
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| Symbol: |
Tg(Igh-Creb1S133A)1Lbox |
| Name: |
transgene insertion 1, Linda M Boxer |
| MGI ID: |
MGI:2447680 |
| Transgene: |
Tg(Igh-Creb1S133A)1Lbox Location: unknown
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| Alliance: |
Tg(Igh-Creb1S133A)1Lbox page
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| Transgene Type: |
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Transgenic (Dominant negative, Inserted expressed sequence) |
| Mutation: |
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Insertion
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Mutation details: An Igh enhancer and a dominant negative form of the Creb1 gene carrying a serine to alanine subsitution at residue 133 (S133A) was used for the transgene. This mutant form of Creb1 cannot be phosphorylated. The Igh enhancer was used to target transgene expression to lymphocytes. Transgene expression was observed in spleen by Northern blot. A low level of transgene expression was also detected in the thymus. Western blot analysis showed a higher amount of Creb1 protein in resting B cells from transgenic mice compared to wild-type. Less phosphorylated Creb1 protein was also observed in transgenic B cells compared to wild-type.
(J:80716)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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| Original: |
J:80716 Zhang CY, et al., Impaired proliferation and survival of activated B cells in transgenic mice that express a dominant-negative cAMP-response element-binding protein transcription factor in B cells. J Biol Chem. 2002 Dec 13;277(50):48359-65 |
| All: |
1 reference(s) |
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Analysis Tools
