Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:80959
Kuo Y, et al., Differential expression of nicotinic acetylcholine receptor subunits in fetal and neonatal mouse thymus. J Neuroimmunol. 2002 Sep;130(1-2):140-54
|
|
Assay type:
|
RT-PCR
|
|
MGI Accession ID:
|
MGI:2653018
|
|
Gene symbol:
|
Chrna3
|
|
Gene name:
|
cholinergic receptor, nicotinic, alpha polypeptide 3
|
|
Probe:
|
Chrna3-pA, Chrna3-pB
|
|
Probe preparation:
|
Antisense labelled with P32 DNA
|
|
Visualized with:
|
Autoradiography
|
|
Assay notes:
|
The PCR products were visualized by Southern blotting.The probe used in this assay was 5' labelled.
|
|
|
Results
|
Image: 1_a3
| Mol. Wt. Marker Lane |
| Lane not used in this assay |
| P adult
|
TS28: brain
|
Present
|
4 µg; total RNA |
C57BL/6J |
| Not Specified |
| Lane not used in this assay |
| Lane not used in this assay |
| Lane not used in this assay |
| Lane not used in this assay |
| Lane not used in this assay |
| Lane not used in this assay |
| Control |
| Mol. Wt. Marker Lane |
| Mol. Wt. Marker Lane |
| Lane not used in this assay |
|