About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:79634 Madan A, et al., Developmental stage-specific expression of the alpha and beta subunits of the HIF-1 protein in the mouse and human fetus. Mol Genet Metab. 2002 Mar;75(3):244-9
Assay type: Northern blot
MGI Accession ID: MGI:2652644
Gene symbol: Hif1a
Gene name: hypoxia inducible factor 1, alpha subunit
Probe: Hif1a cDNA5
Probe preparation: Antisense labelled with P32 DNA
Visualized with: Phosphorimaging
Assay notes: This was a slot blot Northern
Results Image: 1
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex
E15 Brain E15.0 (a) TS23: brain Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
E17 Brain E17.0 (a) TS25: brain Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
E19 Brain E19.0 (a) TS26: brain Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
Day 1 Brain P1 TS27: brain Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
Day 3 Brain P3 TS27: brain Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
Adult Brain P adult TS28: brain Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
Anemic adult Brain Treated sample: no data stored
E15 Heart E15.0 (a) TS23: heart Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
E17 Heart E17.0 (a) TS25: heart Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
E19 Heart E19.0 (a) TS26: heart Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
Day 1 Heart P1 TS27: heart Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
Day 3 Heart P3 TS27: heart Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
Adult Heart P adult TS28: heart Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
Anemic adult Heart Treated sample: no data stored
E15 Kidney E15.0 (a) TS23: metanephros Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
E17 Kidney E17.0 (a) TS25: brain Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
E19 Kidney E19.0 (a) TS26: metanephros Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
Day 1 Kidney P1 TS27: metanephros Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
Day 3 Kidney P3 TS27: metanephros Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
Adult Kidney P adult TS28: metanephros Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
Anemic adult Kidney Treated sample: no data stored
E15 Liver E15.0 (a) TS23: liver Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
E17 Liver E17.0 (a) TS25: liver Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
E19 Liver E19.0 (a) TS26: liver Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
Day 1 Liver P1 TS27: liver Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
Day 3 Liver P3 TS27: liver Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
Adult Liver P adult TS28: liver Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
Anemic adult Liver Treated sample: no data stored
E15 Lung E15.0 (a) TS23: lung Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
E17 Lung E17.0 (a) TS25: lung Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
E19 Lung E19.0 (a) TS26: lung Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
Day 1 Lung P1 TS27: lung Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
Day 3 Lung P3 TS27: lung Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
Adult Lung P adult TS28: lung Present 20 µg; total RNA involves: C57BL * Swiss Webster Not Specified
Anemic adult Lung Treated sample: no data stored
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression was present in all organs at all stages examined. Expression was highest in the brain, followed by heart, kidney, lung, and liver. Expression was higher in the fetal and newborn mice when compared with the adult.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
01/06/2026
MGI 6.24 		The Jackson Laboratory