Kcne1 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:20434 Felipe A, et al., Differential expression of Isk mRNAs in mouse tissue during development and pregnancy. Am J Physiol. 1994 Sep;267(3 Pt 1):C700-5
Assay type:	Northern blot
MGI Accession ID:	MGI:2652593
Gene symbol:	Kcne1
Gene name:	potassium voltage-gated channel, Isk-related subfamily, member 1

Probe:	Kcne cDNA2
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Phosphorimaging
Assay notes:	Authors detailed expression in relative arbitrary units from 0 to 25.

Results

Image: 4A

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	3.4 kb	0.9 kb	Amount	Genetic Background	Mutant Allele(s)	Sex
Adult	P adult (a)	TS28: metanephros	Present (b)	Present (c)	15 µg; total RNA	(C57BL/6J x DBA/2J)F1		Not Specified
F15	E15.5 (a)	TS23: metanephros	Present (d)	Present (e)	15 µg; total RNA	(C57BL/6J x DBA/2J)F1		Not Specified
F19	E19.5 (a)	TS26: metanephros	Present (f)	Present (d)	15 µg; total RNA	(C57BL/6J x DBA/2J)F1		Not Specified
N1	P1 (a)	TS27: metanephros	Present (g)	Present (h)	15 µg; total RNA	(C57BL/6J x DBA/2J)F1		Not Specified
N2	P2 (a)	TS27: metanephros	Present (i)	Present (d)	15 µg; total RNA	(C57BL/6J x DBA/2J)F1		Not Specified
N7	P7 (a)	TS28: metanephros	Present (j)	Present (f)	15 µg; total RNA	(C57BL/6J x DBA/2J)F1		Not Specified
N14	P14 (a)	TS28: metanephros	Present (k)	Present (f)	15 µg; total RNA	(C57BL/6J x DBA/2J)F1		Not Specified
N21	P21 (a)	TS28: metanephros	Present (l)	Present (m)	15 µg; total RNA	(C57BL/6J x DBA/2J)F1		Not Specified

Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) Expression level was 17.5 arbitary units.
(c) Expression level was 9 arbitary units.
(d) Expression level was 2 arbitary units.
(e) Expression level was 1 arbitary unit.
(f) Expression level was 5 arbitary units.
(g) Expression level was 4.5 arbitary units.
(h) Expression level was 3 arbitary units.
(i) Expression level was 3.5 arbitary units.
(j) Expression level was 6 arbitary units.
(k) Expression level was 12.5 arbitary units.
(l) Expression level was 21 arbitary units.
(m) Expression level was 8.5 arbitary units.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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