Tg(Prnp)E11Cwe
Transgene Detail
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| Symbol: |
Tg(Prnp)E11Cwe |
| Name: |
transgene insertion E11, Charles Weissmann |
| MGI ID: |
MGI:2446646 |
| Synonyms: |
E11 |
| Transgene: |
Tg(Prnp)E11Cwe Location: unknown
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| Alliance: |
Tg(Prnp)E11Cwe page
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(Prnp)E11Cwe expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Note |
| mouse |
Prnp (MGI:97769) |
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Mutation details: A transgenic construct was engineered to contain murine Prnp lacking sequence encoding amino acids 32 through 121. Sequence, including that encoding the amino-terminal signal sequence, necessary for proper transport to the endoplasmic reticulum and proper processing was left intact. Transgenic expression was verified by Western blot analysis. Only after treatment with PNGase did the transgenic protein migrate a distance corresponding to the deletion, indicating in vivo glycosylation. A carboxy terminal cleavage product was also detected. The murine Prnp promoter drove expression of similar transgenes (lines 11 and 12) the forebrain and cerebellum. Transgenic transcript was identified in all layers of the cerebellum except for Purkinje cells.
(J:47034)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Nuclear injections were made in zygotes carrying Prnptm1Cwe.
The founder line contained approximately 70 copies of the transgenic construct.
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| Original: |
J:47034 Shmerling D, et al., Expression of amino-terminally truncated PrP in the mouse leading to ataxia and specific cerebellar lesions. Cell. 1998 Apr 17;93(2):203-14 |
| All: |
1 reference(s) |
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Analysis Tools
