Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:81328
Natale DR, et al., Rac-1 and IQGAP are potential regulators of E-cadherin-catenin interactions during murine preimplantation development. Mech Dev. 2002 Dec;119 Suppl 1:S21-6
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:2652275
|
|
Gene symbol:
|
Rac1
|
|
Gene name:
|
Rac family small GTPase 1
|
|
Antibody:
|
anti-rac 1
|
|
Detection system:
|
Secondary antibody coupled to fluorescein
|
|
Assay notes:
|
At least five embryos at each stage were stained. Embryos were viewed using a confocal microscope.
|
|
|
Results
|
Specimen
3a:
postnatal
(more  )
Note: Mature oocyte.
Specimen
3a:
(close  )
|
Genetic Background:
|
CD-1
|
|
Age:
|
postnatal
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Female
|
|
Type:
|
optical section
|
|
Note:
|
Mature oocyte.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
|
3a
|
Expression was present in the cytoplasm and in the cell margins. There was also some punctate staining near the cell membrane.
|
Specimen
3b:
embryonic day 1.0
(more )
Note: Two-cell embryo.
Specimen
3b:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 1.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Note:
|
Two-cell embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
Regionally restricted |
3b
|
Expression was adjacent to the cell membrane encircling each blastomere. There was puncate vesicular staining in the cytoplasm near the cell margins. There was also faint nuclear staining.
|
Specimen
3c:
embryonic day 2.0
(more )
Note: Four-cell embryo.
Specimen
3c:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 2.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Note:
|
Four-cell embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 4-cell stage embryo |
Present |
Regionally restricted |
3c
|
Expression was adjacent to the cell membrane encircling each blastomere. There was puncate vesicular staining in the cytoplasm near the cell margins. There was also faint nuclear staining.
|
Specimen
3d:
embryonic day 2.5
(more )
Note: Compacted 8-cell embryo.
Specimen
3d:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 2.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Note:
|
Compacted 8-cell embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS3: compacted morula |
Present |
Spotted |
3d
|
Punctate staining was seen in the cytoplasm, primarily around the cell margins of each blastomere.
|
Specimen
3e:
embryonic day 2.5
(more )
Note: Compacted 8-cell embryo.
Specimen
3e:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 2.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Note:
|
Compacted 8-cell embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS3: compacted morula |
Present |
Spotted |
3e
|
Punctate staining was seen in the cytoplasm, primarily around the cell margins of each blastomere.
|
Specimen
3f:
embryonic day 3.0
(more )
Note: Morula.
Specimen
3f:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 3.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Note:
|
Morula.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Present |
Spotted |
3f
|
There was punctate staining in the cytoplasm near the nucleus. Staining was absent from the cell margins.
|
Specimen
3g:
embryonic day 4.0
(more )
Note: Blastocyst.
Specimen
3g:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 4.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Note:
|
Blastocyst.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS5: mural trophectoderm |
Present |
Spotted |
3g
|
There was punctate staining the cytoplasm. This staining exhibted a more apical localization in polar trophectoderm cells when compared to mural trophectoderm cells.
|
| TS5: polar trophectoderm |
Present |
Spotted |
3g
|
There was punctate staining the cytoplasm. This staining exhibted a more apical localization in polar trophectoderm cells when compared to mural trophectoderm cells.
|
| TS5: inner cell mass |
Present |
Spotted |
3g
|
There was punctate staining in the cytoplasm.
|
|
Analysis Tools