Kdm5d Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:76911 Xu J, et al., Sex differences in sex chromosome gene expression in mouse brain. Hum Mol Genet. 2002 Jun 1;11(12):1409-19
Assay type:	Northern blot
MGI Accession ID:	MGI:2651498
Gene symbol:	Kdm5d
Gene name:	lysine demethylase 5D

Probe:	Smcy probe1
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Phosphorimaging

Results

Image: 4_Smcy

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	8.4 kb (a)	5.3 kb (a)	Amount	Genetic Background	Mutant Allele(s)	Sex
13.5 dpc M Brain	E13.5	TS21: brain	Present	Present	4-5 µg; poly-A+ RNA	C57BL/6J		Male
13.5 dpc F Brain	E13.5	TS21: brain	Absent (b)	Absent (b)	4-5 µg; poly-A+ RNA	C57BL/6J		Female
Adult M Brain	P adult	TS28: brain	Present	Present	4-5 µg; poly-A+ RNA	C57BL/6J		Male
Adult F Brain	P adult	TS28: brain	Absent (b)	Absent (b)	4-5 µg; poly-A+ RNA	C57BL/6J		Female
Adult M Heart	P adult	TS28: heart	Present	Present	4-5 µg; poly-A+ RNA	C57BL/6J		Male
Adult M Kidney	P adult	TS28: metanephros	Present	Present	4-5 µg; poly-A+ RNA	C57BL/6J		Male
Adult M Liver	P adult	TS28: liver	Present	Present	4-5 µg; poly-A+ RNA	C57BL/6J		Male

Notes:
(a) Expression was higher in embryonic brains than in adult and was higher in adult brain than in other tissues.
(b) Trace hybridization in female lane was due to cross-hybridization of the probe with a homolog.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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