Gjd2 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:47973 Sohl G, et al., The murine gap junction gene connexin36 is highly expressed in mouse retina and regulated during brain development. FEBS Lett. 1998 May 22;428(1-2):27-31
Assay type:	Northern blot
MGI Accession ID:	MGI:2451145
Gene symbol:	Gjd2
Gene name:	gap junction protein, delta 2

Probe:	Cx36 cDNA1
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Autoradiography

Results

Image: 4_right blot

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	2.9 kb	Amount	Genetic Background	Mutant Allele(s)	Sex
brain, 14dpc	E14.0 (a)	TS22: brain	Present (b)	20 µg; total RNA	CD-1		Not Specified
brain, 16dpc	E16.0 (a)	TS24: brain	Present (b)	20 µg; total RNA	CD-1		Not Specified
brain, 18dpc	E18.0 (a)	TS26: brain	Present (b)	20 µg; total RNA	CD-1		Not Specified
brain, P0	P0	TS27: brain	Present (b)	20 µg; total RNA	CD-1		Not Specified
brain, P3	P3	TS27: brain	Present (b)	20 µg; total RNA	CD-1		Not Specified
brain, P7	P7	TS28: brain	Present (b)	20 µg; total RNA	CD-1		Not Specified
brain, P14	P14	TS28: brain	Present (c)	20 µg; total RNA	CD-1		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) After standardization with a GAPDH probe, there is a gradual increase beginning at 20% at dpc14, and reaching 100% expression at P7.
(c) Subsequently expression declined to 40% and reached a basal value of about 8% in adult brain.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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