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Gene Expression Data
Assay Details
Assay
Reference: J:31483 Park CH, et al., Expression of multiple forms of 3 beta-hydroxysteroid dehydrogenase in the mouse liver during fetal and postnatal development. Mol Cell Endocrinol. 1996 Feb 5;116(2):157-64
Assay type: RT-PCR
MGI Accession ID: MGI:2451010
Gene symbol: Hsd3b5
Gene name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 5
Probe: primer pair 2
Visualized with: Ethidium bromide
Assay notes: The primers used amplified two family members. (This amplicon is present at 649 bp.) Individual family members distinguished by subjecting the amplified fragment to restriction enzyme digestion. Each analysis represents results from at least three separate tissue samples at each age.
Results Image: 4b
 Sample Information Bands Other Sample Information
Lane AgeStructure 649 bp Amount Genetic BackgroundMutant Allele(s)Sex
40 U Control
40 P Lane not used in this assay
40 D P40 TS28: liver Present 0.2 µg; total RNA C57BL/6J Male
50 U Control
50 P Lane not used in this assay
50 D P50 TS28: liver Present 0.2 µg; total RNA C57BL/6J Male
V U Control
V P Control
V D Control
IV U Control
IV P Control
IV D Control
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory