About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:31483 Park CH, et al., Expression of multiple forms of 3 beta-hydroxysteroid dehydrogenase in the mouse liver during fetal and postnatal development. Mol Cell Endocrinol. 1996 Feb 5;116(2):157-64
Assay type: RT-PCR
MGI Accession ID: MGI:2451009
Gene symbol: Hsd3b4
Gene name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 4
Probe: primer pair 2
Visualized with: Ethidium bromide
Assay notes: The primers used amplified two family members. (This amplicon is present at 649 bp.) Individual family members distinguished by subjecting the amplified fragment to restriction enzyme digestion. Each analysis represents results from at least three separate tissue samples at each age.
Results Image: 4b
 Sample Information Bands Other Sample Information
Lane AgeStructure 649 bp Amount Genetic BackgroundMutant Allele(s)Sex
40 U Control
40 P P40 TS28: liver Absent 0.2 µg; total RNA C57BL/6J Male
40 D Lane not used in this assay
50 U Control
50 P P50 TS28: liver Absent 0.2 µg; total RNA C57BL/6J Male
50 D Lane not used in this assay
V U Control
V P Control
V D Control
IV U Control
IV P Control
IV D Control
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory