Hsd3b1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:31483 Park CH, et al., Expression of multiple forms of 3 beta-hydroxysteroid dehydrogenase in the mouse liver during fetal and postnatal development. Mol Cell Endocrinol. 1996 Feb 5;116(2):157-64
Assay type:	RT-PCR
MGI Accession ID:	MGI:2451002
Gene symbol:	Hsd3b1
Gene name:	hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1

Probe:	primer pair 1
Visualized with:	Ethidium bromide
Assay notes:	The primers used were designed to amplify all family members. (This amplicon is present at 629 bp.) Individual family members were identified by subjecting the amplified fragment to restriction enzyme digestion. The amount of undigested cDNA remainingis an indication of the proportion of the other isoforms present. Each analysis represents results from at least three separate tissue samples at each age.

Results

Image: 2_lower

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	629 bp	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
13.5 U	Control
13.5 D	E13.5	TS21: liver	Present	.2 µg; total RNA	C57BL/6J		Female
15.5 U	Control
15.5 D	E15.5	TS23: liver	Present	.2 µg; total RNA	C57BL/6J		Female
17.5 U	Control
17.5 D	E17.5	TS25: liver	Present	.2 µg; total RNA	C57BL/6J		Female
1 U	Control
1 D	P1	TS27: liver	Present	.2 µg; total RNA	C57BL/6J		Female
7 U	Control
7 D	P7	TS28: liver	Present	.2 µg; total RNA	C57BL/6J		Female
10 U	Control
10 D	P10	TS28: liver	Present	.2 µg; total RNA	C57BL/6J		Female
16 U	Control
16 D	P16	TS28: liver	Present	.2 µg; total RNA	C57BL/6J		Female	There was a sharp decrease in expression between postnatal days 16 and 20.
20 U	Control
20 D	P20	TS28: liver	Present	.2 µg; total RNA	C57BL/6J		Female
30 U	Control
30 D	P30	TS28: liver	Absent	.2 µg; total RNA	C57BL/6J		Female
40 U	Control
40 D	P40	TS28: liver	Absent	.2 µg; total RNA	C57BL/6J		Female
50 U	Control
50 D	P50	TS28: liver	Absent	.2 µg; total RNA	C57BL/6J		Female

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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